A U-2 OS cell line was sourced from AstraZeneca’s Global Cell Bank (ATCC Cat# HTB-96). Cells were maintained in continuous culture in McCoy’s 5A media (#26600023 Fisher Scientific, Loughborough, UK) containing 10% (v/v) fetal bovine serum (Fisher Scientific, #10270106) at 37 °C, 5% (v/v) CO2, 95% humidity. At … See more All chemical compounds were sourced internally through the AstraZeneca Compound Management Group and prepared in stock … See more Microplates were imaged on a CellVoyager CV8000 (Yokogawa, Tokyo, Japan) using a 20 × water-immersion objective lens (NA … See more The Cell Painting protocol was applied according to the original method1 with minor adjustments to stain concentrations. Hank’s balanced salt solution (HBSS) 10× was sourced from … See more The selected images were bilinearly downscaled from 1996 × 1996 to 998 × 998 pixels to reduce computational overheads. Global intensity normalization was implemented to eliminate intensity differences between … See more WebAnalytical & Enterprise Solutions. An Analytical and Enterprise solutions business, including leading OneSource laboratory services, focused on accelerating scientific outcomes. Close. Life Sciences & Diagnostics.
Image-based profiling for drug discovery: due for a machine …
WebJun 15, 2024 · Cell Painting is a high-content image-based assay applied in drug discovery to predict bioactivity, assess toxicity and understand mechanisms of action of chemical and genetic perturbations. We investigate label-free Cell Painting by predicting the five fluorescent Cell Painting channels from brightfield input. WebJul 14, 2024 · In the context of drug discovery, cell-based phenotypic assays are compound screening systems focused on the modulation of a cell’s phenotype, in a way … hilton hotels happy guarantee
Cell Painting By Numbers
WebUnknown data - compounds with unknown function and the same features extracted from Cell Painting images. The goal is to use the reference data with their known function to … WebAug 25, 2016 · Cell Painting is a high-content screening assay that uses multiplexed fluorescent dyes for image-based profiling of ∼1,500 morphological features. ... specifically the Drug Discovery Core (B ... hilton hotel shanghai pudong