Webfrom culture flasks using trypsin/EDTA (Hyclone). To induce differentiation, growth media were replaced by low-serum media (supplemented with 2% FBS instead of 10% FBS). 2.2. GelMA Preparation. A GelMA lyophilizate and a LAP photoinitiator (PI) were both purchased from CELLINK, Sweden. A Web2.血浆：用edta或肝素作为抗凝剂采集标本，并将标本在采集后的30分钟内于2-8℃ 1000×g离心15分钟，取上清即可检测，或将上清置于-20℃或-80℃保存，但应避免反复冻融。

Profiling chromatin states using single-cell itChIP-seq - Nature

Weband were treated with trypsin/EDTA-HBSS solution (Gibco) for 12min at 37°C, followed by addition of Dulbecco’s modified Eagle’s medium (Gibco) with 10% fetal bovine serum to stop trypsin reaction. The supernatants were removed, and the cortices were re-suspendedwithneurobasalmedium(Invitrogen)con-taining 2% B27 supplement … WebExplore the Hyclone™ products from Cytiva. Treat your cells to a liquid lunch. To say culturing cells for therapeutic treatments is important is an understatement. scottish widows fund charges list

Modeling neural circuit, blood–brain barrier, and myelination …

WebYes, I am using Trypsin with EDTA (Hyclone, to be exact). I adopted two of the techniques for the stubborn cells, and it seems to be working. I am now doing a wash with HBSS (I … WebPBS/0.5 mM EDTA, and incubate at room temperature until cells detach (do not exceed 5-10 minutes). Add 9 ... HAM’s F-12 Hyclone SH30026.02 DMEM Hyclone SH30022.02 Advanced DMEM/F12 (serotonin receptors) Invitrogen 12634-010 … Web10X, sterile-filtered, BioReagent, suitable for cell culture, 5.0 g porcine trypsin and 2 g EDTA, 4Na per liter of 0.9% sodium chloride View Price and Availability Sigma-Aldrich scottish widows fixed interest pension fund