2024 Hygromycin b resistance cassette

Hygromycin b resistance cassette

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August undefined, 2024

Web13 jan. 2024 · All mutants showed similar mCherry expression (Fig. 4A and B) and stable integration of the construct was verified by Southern Blot, detecting the hygromycin resistance cassette (Fig. 4C). Due to the linearization with SmaI of the construct prior to transformation and the digestion of gDNA with EcoRV, bands with sizes of more than … Web24 dec. 2024 · For gene deletion, we provide five different donor vectors for selection marker cassettes. These include standard cassettes for hygromycin B, nourseothricin and …

Three novel antibiotic marker cassettes for gene disruption and …

WebHygromycin B. Hygromycin B interacts with 16S rRNA in a manner different from that of paromomycin, streptomycin, or spectinomycin. 248 Hygromycin B binds close to the A site near the top of helix 44 of 16S rRNA, 191, 198, 228 with one ring ... Here, the marker corresponds to the hygromycin resistance cassette ... Web1 dec. 2010 · Complementation of mutants was performed using hygromycin B resistance, utilizing the pYM70 plasmid (54) and generating a cassette with the HYG resistance gene at the tail end of the PCR product (52). subs lexington ky

Hygromycin Resistance Cassette TaKaRa Bioz

WebA binary vector containing a hygromycin B resistance cassette was introduced into A. tumefaciens, and the resultant strain was co-cultivated with fungal small conidia. … Web1 okt. 1991 · Hygromycin resistance gene cassettes were designed to facilitate vector construction for plant transformation. Unique EcoRI, Pstl, and Sacll sites in the coding … paintbox scratch project

Golden Gate vectors for efficient gene fusion and gene deletion in ...

What is a sequence of hygromycin B resistance gene?

Web26 jan. 2024 · Primary transformants were crossed with the color spore mutant fus1-1 (S23442) and single-spore isolates (ΔSmlon2) with hygromycin resistance were selected . The absence of the Smlon2 gene and the integration of the hph -cassette at the desired locus was verified using primer pairs lon2-ko-v5f/tC1_0 (1400 bp), h3_0/lon2-ko-v3r … Webhygromycin B 70-O-kinase or simply hygromycin phosphotrans-ferase [14]. The specific modification of hygromycin B is a phosphorylation at the 70-OH of the destomic acid ring [15]. Resistance to apramycin (AprR) is conferred by the 777 bp aac(3)IV aminoglycoside 3-N-acetyltransferase gene [13]. Specifically, the subs leaderboard for twitchWebArticle Snippet: The hygromycin B resistance cassette was amplified through PCR from the pCB1004 vector (Carroll et al ., ) using the Gibson assembly primer couple GibFor … sub slab suction radon

"WebHygromycin B is produced by Streptomyces hygroscopicus, a bacterium isolated in 1953 from a soil sample. Resistance genes were discovered in the early 1980s. Mechanism of … " - Hygromycin b resistance cassette

Hygromycin b resistance cassette

pSELECT-hygro : Dual expression cassette plasmid with hygromycin …

Webresistance cassette against hygromycin B with PGK Promoter. Use. Synthetic Biology Tags. Expression. Mutation. Promoter. Availability. Academic Institutions and Nonprofits only Enlarge pSH231-EF1-GFP-HYGRO. Plasmid #115144. Purpose. Safe harbor site 231 knock-in vector with GFP ... WebName Vector Type Resistance Marker Bacterial Resistance Source Sequence Available; 7749 Bicistronic_ires_hygro: Retroviral: Hygromycin: Addgene: bRA66: Yeast Expression, CRISPR, Synthetic Biology

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Web1 okt. 1991 · Hygromycin resistance gene cassettes were designed to facilitate vector construction for plant transformation. Unique Eco RI, Pst l, and Sac ll sites in the coding sequence of a hygromycin B phosphotransferase gene ( … WebExpression plasmid with hygromycin resistance gene. 20 µg . pseth-mcs. TDS MSDS +-$535.00 Add to favorite. pSELECT-hygro-LacZ Unit size Cat. code Docs Qty ... the two expression cassettes are oriented in convergent directions ... Hygromycin B Gold . Selective antibiotic for the hph gene ...

WebVersatile co-expression of graft-protective proteins using 2A-linked cassettes . × Close Log In. Log in with Facebook Log in with Google. or. Email. Password. Remember me on this computer. or reset password. Enter the email address you signed up with and we'll email ... WebThe hygromycin-resistance cassette hygroR, containing hph gene under the control of N. crassa pCPC1 and A. nidulans tTrpC, was amplified by PCR using primer pair neoHY-8 …

Web1 okt. 1999 · Two plasmids are constructed that can be used for cloning as templates for PCR- -based gene disruption, mutagenesis and the construction of DNA chromosome translocation cassettes, and are the first vectors that confer resistance to ampicillin, kanamycin and hygromycin B in bacteria, and to geneticin and hygein in … WebPromega hygromycin b resistance gene cassette Hygromycin B Resistance Gene Cassette, supplied by Promega, used in various techniques. Bioz Stars score: 86/100, …

Web31 okt. 2024 · By combining split points, we create 3- and 6-split Hygromycin resistance genes, ... by removing Puromycin resistance gene and inserting IRES-fluorescent genes downstream of the Gateway cassette.

Web1 jan. 2010 · Gene-specific knockout cassettes should be designed to have 1-1.3 kb of gene-specific 5′ and 3′ flanks and a selectable marker (the hygromycin B phosphotransferase gene, hph, in our case). To ensure appropriate expression of the selectable marker, we use the A. nidulans promoter trpC ( 13 ). subs live countWeb2 feb. 2024 · The cassette includes the FRT site (red), promoter site for hygromycin (green), and the hygromycin resistance gene (blue). (DOCX) History 2024-02-02 - First … subslingers azWebWe have utilized the promoter cassettes to express the hygromycin-resistance gene either from the CaMV 35S or the CaMV 19S promoters, with both chimeric resistance … subs live oak flWeb2 feb. 2024 · Sequence of hygromycin resistance cassette for mutant generation. The cassette includes the FRT site (red), promoter site for hygromycin (green), and the hygromycin resistance gene (blue). (DOCX) Browse. Search. Explore more content. ppat.1009291.s004. docx (20.59 kB) File info Download file. subs learningWebWe have created three new dominant drug resistance cassettes by replacing the kanamycin resistance (kan(r)) open reading frame from the kanMX3 and kanMX4 … paintbox simply super chunkyWebHygromycin B. 41,42 Hygromycin B (Figure 8), produced by S. hygroscopicus, exhibits activities against both prokaryotic and eukaryotic cells. From: Comprehensive Medicinal … paintbox simply chunkyWebHygR 345 .. 1373 = 1029 bp 342 amino acids = 38.1 kDa Product: aminoglycoside phosphotransferase from E. coli confers resistance to hygromycin HygR 345 .. 1373 = … subs lexington sc