WebbThe kit is suitable for synthesis of cDNA up to 13 kb. RiboLock RNase Inhibitor, supplied with the kit, effectively protects RNA templates from degradation. The RevertAid First Strand cDNA Synthesis Kit is supplied with both oligo (dT) 18 and random hexamer primers. The oligo (dT) 18 primer anneals selectively to the poly (A) tail of mRNA. WebbThermo Scientific™ RevertAid™ Reverse Transcriptases are based on M-MuLV enzyme and offer routine cDNA synthesis performance in molecular biology applications. For reliable RNA protection Thermo Scientific™ RiboLock™ RNase Inhibitor is an engineered thermostable enzyme that inhibits the activity of RNases A, B, and C.
RiboLock RNase Inhibitor (40 U/µL) - Thermo Fisher …
WebbThermo Scientific RiboLock RNase Inhibitor #_ _ Lot: _ Expiry Date: _ Concentration: 40 u/µl . Store at -20°C . In total _ vials. www.thermoscientific.com/onebio. Description. Thermo … Webbiptase and Thermo Scientific RiboLock RNase Inhibitor. The recombinant RiboLock ™ RNase Inhibitor ef fectively protects RNA template from degradation by RNases A, B and C at te mperatures up to 55°C. 5X Reaction Mix contains the remaining reaction components: reaction buffer, dNTPs, oligo (dT) 18 and random hexamer primers. Water, nuclease-free name something that shines
Thermo Scientific molecular biology workflow solutions
WebbThermo Scientific RiboLock RNase Inhibitor inhibits the activity of RNases A, B and C by binding them in a noncompetitive mode at a 1:1 ratio. It does not inhibit eukaryotic … WebbThermo Scientific RiboLock RNase Inhibitor (#EO0381) 1.25 µl (50 u) T7 RNA Polymerase 30 u DEPC-treated water (#R0601) to 50 µl 3. Incubate at 37°C for 2 hours. 4. Optional: To remove template DNA add 2 µl (2 u) of DNase I, RNase-free (#EN0521), mix and incubate at 37 °C for 15 min. 5. Inactivate DNase I by phenol/chloroform extraction. Note Webb15 feb. 2024 · Seven Ribonucleases (RNases) have been reported to degrade linear RNAs, including RNase II, RNase R, and polynucleotide phosphorylase (PNPase). Recently, a strong 3′ to 5′ exonuclease, RNase R, was widely used to enrich the circRNAs and intronic lariats, both of which are resistant to RNase R because they lack of 3′ overhangs [32], [33]. name something that people grow fields of